Thapsigargin

Описание:

Thapsigargin, a guaianolide-type sesquiterpene lactone, is a widely used inhibitor of the ubiquitous sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA). It can activate endoplasmic reticulum (ER) stress and the unfolded protein response (UPR). Thapsigargin is a natural phytochemical found in the roots and fruits of Mediterranean plants from Thapsia garganica (Apiaceae) species. Thapsigargin promotes the release of translational arrest and induces proteome-wide changes in metabolic pathways within cells infected with coronavirus (CoV).

Применение:

Thapsigargin has been used as an endoplasmic reticulum (ER) stress inducer:to study its effect on cyclase-associated protein 2 (CAP2) expression in liver cancer cellsin the cell viability experiment using ARPE -19 cells (human retinal pigment epithelial cells)to study its effect on lipocalin 2 (LCN2) glycosylation in primary hepatocytesto study the consequences of calcium depletion in the endoplasmic reticulum (ER) of cellsto study its effect on tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA expression in rat Sertoli cells